Are you new to music production outside the box (meaning not on a computer)?
It depends on what you want to do . This will determine the setup.
The Audiofuse is a Swiss Army knife and can be used in lots of ways, depdnding on what you are looking to get from it.
I mostly use it for midi and for the XLR preamps into an akai mpc live. I have tons of outboard equipment.
First get familiar with the operation of the Audiofuse. Form that point it wil become clear what you need to do to allow it to work best in your setup.